Frequently Asked Questions
SAMPLING SITES
How were the sites chosen?
Sampling sites for The Great Australian Platypus Search were selected in consultation with Odonata, La Trobe University, EnviroDNA and DELWP. These sites were chosen using a rigorous sampling methodology to ensure a representative sample of the state, as many variables as possible were considered.
My local waterway doesn’t have a sampling site on the map? Has it been missed?
The sampling design covers a representative sample of Victorian waterways so that means nearly all are being sampled. However, when sites are reserved they are removed from the map.
We have also offered school groups across the state a selection of sites near them. If they do not take these sites up, they will be added to the map on our website and offered to the public. When new sites are available we will announce this via the Great Australian Platypus Search Facebook Group.
Will more sites be added?
At this stage it is unlikely that more sites will be added, however if new sites are announced this will be announced via the Great Australian Platypus Search Facebook Group.
SAMPLING
When do my samples need to be done by?
The Great Australian Platypus Search is gathering data in Victoria during the 2021 platypus breeding season, which generally runs from the beginning of August to the end of November. Therefore, you need have completed your samples by the end of November.
Can anyone participate in eDNA sampling?
EnviroDNA has designed eDNA water sampling methods that are simple enough for the non-expert to implement with no previous experience. After a quick training session, community members across most age groups are fully capable to undertake eDNA sampling.
What’s involved in collecting eDNA samples?
The eDNA samples are collected using an eDNA filter kit, provided by EnviroDNA. Citizen scientists will head down to prearranged local waterway sampling sites, collect the eDNA samples and then send them back to EnviroDNA’s laboratory for processing.
What is included in the eDNA kit?
1 x Pair of latex gloves
2 x Filter units
1 x Disposable syringe
1 x Zip lock pouch (BioPE Recyclable) with sample label
1 x 100% certified home compostable mailer bag
2 x Small syringes with sample preservative
How do you use an eDNA filter kit?
For specific instructions on how to use an eDNA filter kit and conduct your sample, please watch The Great Australian Platypus Search: eDNA Water Sampling Tutorial and Safety Video.
How much water do we have to filter?
There is no fixed amount of water to filter for each sample and it will vary significantly depending on water quality (i.e. sediment, algae). The more water you can filter, the better chance of detecting the target DNA so filter as much water as possible. Push water through the filter using the syringe until you are unable to continue (typically 100-500 mL - remember to keep track of how much water you have filtered!). Do not hurt yourself trying to force the water through the syringe.
Where should I take the water sample from (i.e. middle of stream, water surface etc)?
Samples are typically taken from the water’s edge and at the surface, as this is most easy to access. Try to take a sample from the clearest water available and avoid sediment that may have been stirred up or algae as this will clog the filter and restrict the volume of water filtered (in some waterways this will be unavoidable). If possible and safe to do so, you may space your samples out across the site to provide more representative samples of the site (i.e. ~20-30m between 1st and 2nd sample). In flowing water, DNA is relatively evenly dispersed but can be more patchy in still water.
Should we take samples if it has just rained and the river is flowing swiftly?
Heavy rainfall can wash sediment into the waterway and make it more difficult to access sites. If you can, please wait a few days after rainfall until the turbidity and flows have returned to normal to take samples.
What species are being surveyed?
This project will be detecting eDNA from all aquatic vertebrates, meaning that scientists will be able to develop a comprehensive cross-section of species living in waterways across the state. So while some samples may not reveal that platypus live in the area being sampled, they will still offer scientists an understanding of what species are there instead.
SAFETY
Is eDNA sampling safe and what are the precautions involved?
eDNA water sampling is relatively simple and safe. In fact, you are not required to enter the waterway at all and should avoid this as it can contaminate the sample. The main risks are accessing the site and general precautions for working outdoors (i.e. sunburn, snakes, insect bites). First, please ensure you have a safe place to park. Then assess the site and determine the safest access to the water’s edge to obtain a sample. Take care of steep banks, uneven ground, slippery rocks or mud, and prickly/thorny vegetation such as blackberries.
What if I can’t get a sample from my allocated site because there is no water or no safe access?
Due to the number of sites and scale of this project (the largest ever attempted!), sampling sites have been selected based on a desktop analysis only and sites have not been ground-truthed. While we hope sites represent permanent waterways and access points (i.e. road crossings), this may not always be the case in reality. There will almost certainly be some sites that are dry or are impossible to access due to steep banks, thick blackberry thickets, road closures etc. Please use your best judgement and if a site doesn't seem safe for any reason, DO NOT PROCEED.
If you are unable to take a sample from the allocated site, first look for safe access for up to 500m up or down stream (please do not enter private property without permission), this will still be considered the same site. If this is still not feasible, use your local knowledge to choose another site on the same waterway within ~5km. Please make sure you note this on your sample notes via the app (app should automatically record your location but confirmation is encouraged).
What type of clothing should I wear?
We recommend wearing comfortable and protective clothing as if you were out bush walking - meaning long pants, sturdy shoes, a hat if needed, etc. Gaiters are also recommended to protect against snakes and prickles/thorns.
Is it safe to go water sampling alone?
Always have a person with you while sampling. While sampling is a safe and easy process, accidents can happen and therefore it is essential you always bring along another person.
Is sampling allowed during COVID-19 restrictions?
Please make sure you are following all relevant COVID-19 restrictions. This includes rules around travel, face masks and restricted activity.
The Department of Health and Human Services (DHHS) has created a COVID-19 Factsheet for volunteering. This factsheet provides information for prospective and current volunteers on the ‘COVID-19 Guidance for coordinators to support volunteers’, and reiterates that organisations are required to implement actions to slow the spread of coronavirus (COVID-19).
If you are unaware of the current restrictions, please check advice from the Victorian Government here.